coli6Nutrient AgarMICROBIOLOGY DEPARTMENT FACULTY OF MEDICINE HASANUDDIN UNIVERSITY MEDIUM AGARMICROBIOLOGY DEPARTMENT FACULTY OF MEDICINE HASANUDDIN UNIVERSITYĥMac Conkey Medium MacConkey MediumUntuk isolasi dan diferensiasi basil enterik Gram-negatif.Komposisi: Enzymatic Digest of Gelatin 17 g Enzymatic Digest of Casein 1.5 g Sumber nitrogen dan vitamin bagi bakteriEnzymatic Digest of Animal Tissue 1.5 g Lactose 10 g Melihat kemampuan bakteri memfermentasikan laktosa PINK COLOURBile Salts Mixture 1.5 g Crystal Violet 0.001 g Agen selektif, menghambat bakteri cocci gram (+)Sodium Chlorida 5 g Mempertahankan lingkungan osmotikNeutral Red 0.03 g Zat warna dasarAgar13.5 g dan Final pH: 7.1 0.2 at 25 oC Meliputi:4Mac Conkey MediumNutrient AgarSS AgarTCBS Agar Mengamati morfologi bakteri Enterobactericea pada medium agar dan interpretasinya.Mengamati karakteristik bakteri Enterobactericea pada Triple Sugar Iron Agar (TSIA) dan interpretasinya.Mengamati hasil tes-tes biokimia bakteri Enterobactericea dan interpretasinya.Mengamati medium transport dan medium enrichment yang digunakan untuk spesimen yang disuspek mengandung bakteri Enterobacteriacea. MICROBIOLOGY DEPARTMENT FACULTY OF MEDICINE HASANUDDIN UNIVERSITYģKegiatan PraktikumMICROBIOLOGY DEPARTMENT FACULTY OF MEDICINE HASANUDDIN UNIVERSITY
PRAKTIKUM PERTUMBUHAN DAN PERKEMBANGAN MIKROBA MAC
Specimen : Depend on symptoms and timingUrine, blood, pus, spinal fluid, sputum, or stool Gram stainingCultures (Blood Agar, EMB, Mac Conkey, SS Agar, TCBS)Biochemical Tests (TSI, SIM, VP, Methyl Red, citrate, Urea, KH test)Nucleic acid methods (PCR, hybridization, blots)Antibody mediated (ELISA, slide/latex agglutination, widal reaction, tagged fluorescence, titer)Diagnosing in Microbiology2Escherichia coli Shigella dysenteriaeSalmonella typhiiVibrio cholera PENJELASAN PRAKTIKUMSISTEM GASTROENTEROHEPATOLOGIMICROBIOLOGY DEPARTMENTFACULTY OF MEDICINE HASANUDDIN UNIVERSITYMAKASSAR 2014ġPENDAHULUANMICROBIOLOGY DEPARTMENT FACULTY OF MEDICINE HASANUDDIN UNIVERSITY
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